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Cells were then blocked with 5% normal swine serum for 1 h at room temperature prior to overnight primary antibody incubation at 4 C using anti-H2AX (1:400

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Parallel immunofluorescence analyses also revealed a similar down-regulation and altered pattern of distribution at cell-cell junction for occludin (Figure 4B)

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In its statement to the Bureau, PMI acknowledged that adult smokers switching to Iqos obtain comparable amounts of nicotine to that from cigarettes